Expression and purification of metalloproteins and their structural characterization by solution NMR

Expression and purification of metalloproteins and their structural characterization by solution NMR Italian Ministry of Education, University and Research Chemistry » Inorganic chemistry The research programm is aimed to characterize by solution NMR spectroscopy, specific metallo-proteins. Recombinant protein expression in E. coli will be exploited to produce selected isotopically enriched in 15N and 15N/13C metallo proteins. Their 3D structure and the interaction with protein...