Post-doctorant en biochimie des métalloprotéines

Biologie, biophysique et biochimie Post-doctorant en biochimie des métalloprotéines H/F Projet REPAROX - PEPR The REPAROX project aims to understand and harness repair mechanisms of [Fe-S] enzymes, key biocatalysts in biotechnology whose production and activity are severely limited under aerobic conditions, and translate this mechanism through an ab initio design approach to iron–sulfur biocatalysts of biotechnological interest.The project builds on the recent discovery of a novel [Fe-S] repair mechanism, involving:. An intrinsic component linked to the structure of the Quinolinate synthase NADA enzyme. An extrinsic component associated with the mitochondrial environmentThe project combines: Genetics, Molecular biology, Structural biology,  Biochemistry, Spectroscopy, Computational protein design, and engineering metabolic pathways in yeastIMPACT: REPAROX aims to overcome a major bottleneck in the aerobic bioproduction of [Fe-S] enzymes, enabling the development of more robust and tailor-made biocatalysts for industrial applications.Within the REPAROX project, the BioCat team in Grenoble aims to biochemically, enzymatically, and spectroscopically characterize NadA from several organisms, both as an isolated enzyme and in complex with the protein partners involved in its repair mechanism under oxidative conditions. Formation : PhD in biochemistryCompétences : The candidates should have a strong expertise in biochemistry and enzymology and an interest for spectroscopy, structural biology and metals in biology. Candidates should preferably have a 2 to 4 years research experienceExpériences et activités sur place : expression and purification of proteins, enzymatic assay, metal content determination, biophysical characterization (SEC-MALLS, CD, Mössbauer and EPR spectroscopy, BLI (protein-protein interaction)). Conformément aux engagements pris par le CEA en faveur de l'intégration des personnes handicapées, cet emploi est ouvert à toutes et à tous. Le CEA propose des aménagements et/ou des possibilités d'organisation pour l'inclusion des travailleurs handicapés